| Benzodiazepines and metabolites | Tested Concentration (ng/mL) | Positive/Negative | Cross-reactivity (%) |
| α-Hydroxyalprazolam | 110 | Positive | 182 |
| α-Hydroxytriazolam | 140 | Positive | 143 |
| Alprazolam | 100 | Positive | 200 |
| 7-Aminoclonazepam | 800 | Positive | 25 |
| 7-Aminoflunitrazepam | 225 | Positive | 89 |
| 7-Aminonitrazepam | 500 | Positive | 40 |
| Bromazepam | 300 | Positive | 67 |
| Chlordiazepoxide | 2000 | Positive | 10 |
| Clobazam | 450 | Positive | 44 |
| Clonazepam | 350 | Positive | 57 |
| Clorazepate | 100 | Positive | 200 |
| Delorazepam | 100 | Positive | 200 |
| Demoxepam | 1500 | Positive | 13 |
| Desalkylflurazepam (Norfludiazepam) | 110 | Positive | 182 |
| Diazepam | 80 | Positive | 250 |
| Estazolam | 115 | Positive | 174 |
| Flunitrazepam | 125 | Positive | 160 |
| Flurazepam | 70 | Positive | 286 |
| Lorazepam | 250 | Positive | 80 |
| Lorazepam glucuronide | 400 | Positive | 50 |
| Lormetazepam | 175 | Positive | 114 |
| Medazepam | 200 | Positive | 100 |
| Nitrazepam | 290 | Positive | 69 |
| Nordiazepam (Desmethyldiazepam) | 70 | Positive | 286 |
| Oxazepam | 200 | Positive | 100 |
| Oxazepam glucuronide | 350 | Positive | 57 |
| Prazepam | 140 | Positive | 143 |
| Temazepam | 130 | Positive | 154 |
| Temazepam glucuronide | 250 | Positive | 80 |
| Triazolam | 90 | Positive | 222 |