Set

Primer name1

Forward (5’→3’)

Reverse(5’→3’)

Product (bp)

Annealing temp. (˚C)

1

5’-flanking region 1

GGCCAGAACCAAGTCCTC

GGTACGTGTCCGAACTCCTC

544

64

2

5’-flanking region 2

TCTTTGTGCGGGCATCAG

CCTGCAAAACTCCTGGGTAG

428

64

3

5’-flanking region 3

GGCAGAGGAGGTTTCGAG

GTAGACCTTGCGATCGATGA

420

58

4

Exon 1

GAAGACAAAAGCCGAAAGGA

CGCTGGAGGTCTTGTCTGA

375

58

5

Exon 2

AGCCGCAGTAGAGGTCTTTG

TTCCTCATCCGGTCAGACTC

181

58

6

Exon 3 and 4

TGACTCCTTCCTCGTGCTCT

GTGCCCACCCACTAACTGAT

500

58

7

Exon 5

CCTGCTCATCCCTGTTCCT

CCCATCCCCTTCATCGTA

175

60

8

Exon 6 and 7

GGGCTCACAGTCTCTGCTTC

CCTCCTCCCCACACTCCT

579

62

9

Exon 8 and 9

GCAAGACACTCCCACGTCTA

TCCTGGGGACATGGACCT

553

58

10

Exon 10

CTTTGTGCTTCTGCTTCCAG

TCCACTGTATGCCCTCCAC

178

64

11

Exon 11

AGAGGCGGACAGGGTCTC

ATGCCCACCCCTCCTTAC

238

62

12

Exon 12 and 3’-untranslated region 1

CATCCATTTCCTCCTCTTCC

GGCCCTGGACATCTGAAG

599

58

13

3’-untranslated region 2

TTCTGTGGGGTGATGATGTG

TTGATAGACCGGGAAAGAGC

555

62

14

3’-untranslated region 3

AGCCCAGCAGCTCATCAC

TTCTCAGTGGGAGGGCTTC

573

62

15

3’-untranslated region 4

CAGCTCTGACTGGTGATGGA

AACGGGAAGTGGAGGAGAAT

244

58