| gene | Primers name | primer sequences | PCR conditions | Product size (bp) |
| 5’ → 3’ | ||||
| SRY 1 | SRY-1 F | CATGAACGCATTCATCGTGTGGTC | 94˚C-2 min (94˚C-1 min, 65˚C-1 min, 72˚C-1 min) | 254 |
| SRY-1 R | CTGCGGGAAGCAAACTGCAATTCTT | ×35 cycles, 72˚C-10 min | ||
| SRY 2 | SRY-2 F | GAATATTCCCGCTCTCCGGAG | 94˚C-2 min (94˚C-1 min, 58˚C-1 min, 72˚C-1 min) | 418 |
| SRY-2 R | ACCTGTTGTCCAGTTGCACT | ×35 cycles, 72˚C-10 min | ||
| XES | XES10 | GGTGTTGAGGGCGGAGAAATGC | 94˚C-5 min (94˚C-1 min, 62˚C-2 min, 72˚C-2 min) | 779 |
| XES11 | GTAGCCATTGTTACCCGATTGTC | ×30 cycles, 70˚C-10 min | ||
| ATL1 | ATL1-F | CCCTGATGAAGA ACT TGTATC TC | 94˚C-2 min (94˚C-1 min, 58˚C-1 min, 72˚C-1 min) | 300 |
| ATL1-R | GAAATTACACAC ATAGGTGGCACT | ×35 cycles, 72˚C-10 min |