| Stains | Spot color | Decision |
| Ninhydrin | Slight pink | Amino groups |
| Rhodamine 6G | Orange | Lipids |
| Fluorescein | Yellow | Simple lipids |
| Dittmer reagent | - | Non phospholipids |
| FeCl2 | Purple | Sterol lipids |
| Hydroxylamine chloride | Slight purple | Ester lipids |