Techniques

Reagents And Their Number

Total Time Required

1

Salting out technique

Digestion Buffer + Proteinase K + Sodium Acetate + Ice Cold 98% Ethanol + Ice Cold 70% Ethanol + 1XTE Or Water

2 hours and 47 min.

2

Chelex technique

Deionize Water + 1XPBS/10% Saponin Solution + 20% W/V Chelex 100 Resin

37 min

3

Chargswitching technique

Chargeswitch Magnetic Beads (negative at low PH and positive at high PH)

10 min

4

prepGEM technique

PCR Graded water + 10× Buffer BLACK + prepGEM

20 min

5

Extraction from preserved specimen (special technique)

Digestion buffer [CaCl₂ + 2% SDS + DTT + proteinase K + Tris buffer + NaCl] + 100% EtOH + phenol chloroform + 100% isopropanol + glycogen + 3 M sodium acetate + 85% ethanol + biology grade H₂O

Overnight step + 2 - 4 h

6

Purgene technique

Lysis Solution + Purgene Proteinase K + RNase A + Protein precipitation solution + Isopropanol

33 - 63 min

7

DNAzol technique

DNAzol Solution + 100% Ethanol + 75% Ethanol + 8 Mm NaOH

10 - 30 min

8

DNeasy technique

ATL Buffer + Proteinase K + Ethanol + AW 1 Buffer + AW 2 Buffer + AE Buffer

1 - 3 h

9

Extraction from Copal (Semi-fossilized insect body)

Pre-extraction treatment solution [30% Bleach + Alcohol+ Chloroform + UV − irradiated HPLC graded water], Extraction Buffer [Tris-HCl + NaCl + CaCl₂ + Dithiothreitol + 2% Sodium dodecyl sulphate + Proeinase K] and then Agitate + QiaQuick PCR kit

Pre-extraction treatment time 2 - 8 days + 20 h

10

CTAB-PVP (Extraction from Xylophagus insects)

Extraction buffer [EDTA + Tris-HCl + NaCl m + 2% w/v CTAB + 4% w/v PVP-40] + 2% v/v β-mercaptoethanol + RNase + Chloroform isoamyl alcohol + 95% ethanol + 70% ethanol + analytical graded water

1 hour and 10 min.