Transgenic event/sample

Annealing and extension

Quantity of primer (nM) forward/reverse

Bt11/corn

at 60˚C/1min and 20s

433/433

TC1507/corn

at 60˚C/1min and 10s

400/400

MIR 162/corn

at 60˚C/1 min

367/367

NK 603/corn

at 60˚C/1min and 30s

333/333

MON810/corn

at 60˚C/1min and 30s

433/433

MON89034/corn

at 60˚C/1min and 30s

500/500

GA21/corn

at 60˚C/30s

433/433

MON88017/com

at 60˚C/1min

200/200

GTS-40-3-2/soybean

at 60˚C/1min

300/300

MON87701/soybean

at 60˚C/1min

200/200

MON89788/soybean

at 60˚C/1min

266/266

MON531/cotton

at 60˚C/50s

267/267

MON1445/cotton

at 60˚C/1min

233/233

LLcoton25/cotton

at 60˚C/1min

133/133

281-24-236 /cotton

at 60˚C/50s

200/200

3006-210-23/cotton

at 60˚C/1min

300/300

GHB 614/cotton

at 60˚C/1min

167/167

T304-40/cotton

at 60˚C/50s

167/167

GHB 119/cotton

at 60˚C/50s

133/133

MON15980/cotton

at 60˚C/1min

266/266

MON88913/cotton

at 60˚C/1min

200/200

Adh/corn

at 60˚C/1min

133/133

Lec/soybean

at 60˚C/1min

333/333

AdhC/cotton

at 60˚C/1min

133/133