| In vitro | In vivo | SEM | P-value | |
Eh1, mV | T0 | –115 | –157 | 7.34 | 0.002 |
| T8 | –189 | –189 | 4.48 | 0.996 |
pH | T0 | 6.91 | 6.66 | 0.03 | <0.0001 |
| T8 | 6.82 | 6.62 | 0.03 | <0.0001 |
rH2 | T0 | 10.0 | 8.1 | 0.28 | <0.0001 |
| T8 | 7.3 | 6.9 | 0.17 | 0.235 |
Total VFA, mM | T0 | 27.9 | 71.5 | 4.51 | <0.0001 |
| T8 | 54.9 | 65.0 | 1.83 | 0.001 |
Acetate, mM | T0 | 20.2 | 51.8 | 3.26 | <0.0001 |
| T8 | 38.1 | 46.5 | 1.32 | <0.0001 |
Propionate, mM | T0 | 4.8 | 12.5 | 0.83 | <0.0001 |
| T8 | 10.4 | 11.4 | 0.34 | 0.049 |
Butyrate, mM | T0 | 2.0 | 5.2 | 0.33 | <0.0001 |
| T8 | 4.6 | 5.6 | 0.17 | 0.001 |