Reagents | rbcL | matK Volume per reaction (µl) | ITS2 |
10% Trehalose | 6.25 | --- | 6.25 |
20% Trehalose | ---- | 1.875 | --- |
ddH2O | 2.00 | 2.60 | 2.00 |
10X Buffer | 1.25 | 0.75 | 1.25 |
50 mM MgCl2 | 0.625 | 0.225 | 0.625 |
10 µM Primer Forward | 0.125 | 0.375 | 0.125 |
10 µM Primer Reverse | 0.125 | 0.375 | 0.125 |
10 mM dNTPs | 0.0625 | 0.15 | 0.0625 |
*DNA Polymerse (5U/µl) | 0.06 | 0.15 | 0.06 |
DNA Template (20 - 40 ng/µl) | 2.00 | 1.0 | 2.00 |
Total Reaction volume | 12.5 | 7.5 | 12.5 |