Cryopreservation treatments | Viability (%) ± S.E. (%) | Germination (%) ± S.E. | |||
LS (min) | PVS2 (min) | −NL | +NL | −NL | +NL |
0 | 0 | 74.0 ± 4.9 | 64.0 ± 4.9 | 92.5 ± 1.6 | 89.5 ± 2.6 |
10 | 0 | 78.0 ± 4.9 | 54.0 ± 4.9 | 81.5 ± 2.9 | 71.4 ± 3.9 |
25 | 68.0 ± 4.9 | 60.0 ± 4.9 | 72.4 ± 4.8 | 67.6 ± 4.2 | |
50 | 72.0 ± 4.9 | 54.0 ± 4.9 | 64.0 ± 5.2 | 73.8 ± 3.1 | |
20 | 0 | 48.0 ± 4.9 | 64.0 ± 4.9 | 73.3 ± 5.5 | 71.9 ± 4.6 |
25 | 64.0 ± 4.9 | 70.0 ± 4.9 | 71.9 ± 4.6 | 78.1 ± 1.7 | |
50 | 62.0 ± 4.9 | 58.0 ± 4.9 | 77.6 ± 7.1 | 70.8 ± 3.2 |