Inoculation

(CFU ml−1)

Boll appearancea

Bacteria recoveredb (LBA)

Bacteria recoveredb (LBA + Rifc)

CC119R

106

No exterior symptoms; entire locule infected and rotted

1 × 1010 CFU/g

1 × 1010 CFU/g

103

No exterior symptoms; entire locule infected and rotted

5 × 108 CFU/g

5 × 108 CFU/g

101

No exterior symptoms; entire locule infected and rotted

5 × 108 CFU/g

5 × 108 CFU/g

CC119Rd (heat-killed)

106

Puncture mark visible; no discoloration of locule

<101 CFU/g

<101 CFU/g

103

Puncture mark visible; no discoloration of locule

<101 CFU/g

<101 CFU/g

101

Puncture mark visible; no discoloration of locule

<101 CFU/g

<101 CFU/g

H2O

Puncture mark visible; no discoloration of locule

<101 CFU/g

<101 CFU/g