Volume of the STOCK aliquot (ml)

Final preparation

Calculated pH

NaH₂PO₄

solution

Na₂HPO₄

solution

Final volume buffer (ml)

Concentration buffer (M)

Obtained

pH

7.51

5

10

25

0.12

7.08

7.30

18

22

50

0.16

6.81

7.21

10

10

50

0.08

6.80

7.10

22

17

100

0.078

6.67

6.91

20

10

100

0.06

6.50