Primer sequence

Annealing temperature (˚C)

Product (bp)

IGF-1

ATTTCAACAAGCCCACAG

TCCCTCTACTTGCGTTCT

56

219

IGF-1R

CCTACAACATCACCGACCCG

CCACGACCCATTCCCAGA

58

464

IGFBP-3

CCCTCTACTTGCTCGATTC

ACGTGCCTACCCACCTTC

54

240

GAPDH

GGAGCCAAACGGGTCATCATCTC

GAGGGGCCATCCACAGTCTTCT

62

233