Reagent

Amount per liter final volume (pH 8)

(NH₄)₂SO₄

100 mg

MgSO₄∙7H₂O

50 mg

CaCl₂∙2H₂O

20 mg

FeSO₄∙7H₂O

1 mg

K₂HPO₄

200 mg

D-glucose or CH₃COONa

500 mg