Protein (mg) recovered per liter bacterial cells

Input

Non-bound

Eluted

% Yield

Expressed in TB1 E. coli

MBP

81.8 ± 4.8

66.52 ± 4.72

0.98 ± 0.02

1.2 ± 0.05

MBP CysRepeat1

174.45 ± 0.45

123.54 ± 1.74

9.48 ± 0.4

5.43 ± 0.21

Expressed in SHuffle E. coli

MBP

84.4 ± 3.1

67.54 ± 1.42

0.85 ± 0.04

1.01 ± 0.01

MBP CysRepeat1

81.25 ± 22

64.12 ± 16.2

2.51 ± 1.01

2.97 ± 0.44