Fractions

Extract solution

Soil (g)

Solution (ml)

Conditions

References

Cold-Water soluble C (CWSC)

Distilled water

3

30

30 min extraction at 20˚C and 20 min centrifugation at 3000 rpm

Ghani et al., 2003

Hot-Water soluble C (HWSC)

Distilled water

3

30

16 hrs extraction at 80˚C and 20 min rotation at 3000 rpm

Ghani et al., 2003

Moderately Labile C (MLF)

125 mM Na₄P₂O₇ (pH 5)

3

25

24 hrs end-over-end rotation (40 rpm) at 25˚C

Erich et al., 2012

Microbial Biomass C (MBC)

0.5 M K₂SO₄

20

100

1-hour shake, sterilization, and extraction

Ghani et al., 2003

Resistant C Fraction (RF)

RF = Total SOC – ${\displaystyle \sum \text{Four}\text{Fractions}}$

This Study