Time (h) | CULTURE | TREATMENT | OBSERVATIONS | |
0 | Asynchronous | Sorbitol | ||
5% - 7%p | At least 1.5% parasitemia after treatment | |||
90% rings | Consider decrease in parasitemia (80%) | |||
5% Hematocrit | ||||
24 | Mature stages | Plasmion | Consider mature stage reinvasion (×4 up to ×9 when synchrony increases) | |
48 | Ring stages | Sorbitol | Consider age of rings | |
(Rings of 0 - 16 h, best results) | ||||
72 | Mature stages | If %p > 2: Plasmion | High parasitemia in mature stages | |
If %p < 2: dilution to adjust to 1% parasitemia | may compromise viability | |||
96 | Rings stages | If %p > 3 dilution: to adjust to 3% parasitemia | The next day will be the same %parasitemia with mature stages | |
120 | Mature stages | Medium change/Plasmion | Depending on degree of synchrony: | |
Plasmion if %mature stages < 90% | ||||
Medium change if synchrony is high | ||||
144 | Ring stages | Sorbitol/Dilution | Sorbitol if there are still mature stages Dilution to keep the culture until assay |