Time (h)

CULTURE

TREATMENT

OBSERVATIONS

0

Asynchronous

Sorbitol

5% - 7%p

At least 1.5% parasitemia after treatment

90% rings

Consider decrease in parasitemia (80%)

5% Hematocrit

24

Mature stages

Plasmion

Consider mature stage reinvasion

(×4 up to ×9 when synchrony increases)

48

Ring stages

Sorbitol

Consider age of rings

(Rings of 0 - 16 h, best results)

72

Mature stages

If %p > 2: Plasmion

High parasitemia in mature stages

If %p < 2: dilution to adjust to 1% parasitemia

may compromise viability

96

Rings stages

If %p > 3 dilution: to adjust to 3% parasitemia

The next day will be the same %parasitemia with mature stages

120

Mature stages

Medium change/Plasmion

Depending on degree of synchrony:

Plasmion if %mature stages < 90%

Medium change if synchrony is high

144

Ring stages

Sorbitol/Dilution

Sorbitol if there are still mature stages

Dilution to keep the culture until assay