SLC26A2 utation^a

Reagents

Forward primer

Reverse primer

Restriction enzyme

c.−26 + 2T > C

5’-CTTCGGAGTC

CGAGCGATGG- 3’

5’-GACCCCTGAT

CTGGGATTCT- 3’

Hph I

c.559 C > T

5’-AGGAAGCTGA

ACCATCTATC- 3’

5’-GATTCCTCAG

ATCCCTTAGAG- 3’

Dde I

c.862 C > T

5’-CTCCATGCAA

GAAATGTCAGG- 3’

5’-TGATACAGTG

ATAGCAAAACC- 3’

Sty I

c.1984 T > A

5’-CAACCCAATC

TTAATAAAGGTG- 3’

5’-CTAGACATTC

TTCTATCTACC- 3’

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