| Biological indicator | Package type | Exposure time (min) | |
| At 50˚C | 30 | 45 | |
| B. atrophaeus ATCC 9372 (1.0 × 106 CFU) | Tyvek package | − | − |
| Glassine | −c | −c | |
| G. stearothermophilus ATCC 7953 (1.3 × 106 CFU) | Tyvek package | − |
|