| 3’ adaptor |
|
| BC8 | CCCTTCTCGTATGCCGTCTTCTGCTTG |
| RA3 | TGGAATTCTCGGGTGCCAAGG |
| 5’ adaptor | |
| PB | rGrUrUrCrArGrArGrUrUrCrUrArCrArGrUrCrCrGrArCrGrArUrC |
| RT primer | |
| RT1 | CAAGCAGAAGACGGCATACGA |
| RT2 | GCCTTGGCACCCGAGAATTCCA |
| Amplification primers |
|
| PB' | AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA |
| Bulging primer | CAAGCAGAAGACGGCATACGANNGAAGGG |
| TruSeq primer I | CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA |