Factors

Coded co-ordinates Optimal values

Real optimal values

Unit

B (pH)

X₅₀ = 1.19

5.68

-

D (starch)

X₁₀ = −0.206

8.97

g/l

E (yeast extract)

X₂₀ = 0.86

2.86

g/l

G (CaCl₂

X₃₀ = 1.12

1.224

g/l

J (salts):

0.6072

g/l

FeSO₄∙7H₂O (25%) =

X₄₀ = 1.48

0.1518

g/l

MnSO₄ (25%) = ¼ [J]

0.1518

g/l

MgCl₂∙6H₂ O (50%) = 1/2 [J]

0.3036

g/l

After optimization

Predicted

Experimental

1267.5 IU

1345, 16 ± 150 IU