| Temperature | Concentration | Inhibition zone size E. coli (mm) | Inhibition zone size S. aureus (mm) |
| 100˚C | C1 | 9.5 | 12.0 |
| C2 | No growth on NPs | 9.5 | |
| C3 | 9.5 | No growth on NPs | |
| C4 | 10.0 | 12.0 | |
| C5 | No growth on NPs | No growth on NPs | |
| 120˚C | C1 | 9.5 | 9.5 |
| C2 | 10.0 | 10.0 | |
| C3 | No growth on NPs | No growth on NPs | |
| C4 | 11.0 | 12.0 | |
| C5 | No growth on NPs | No growth on NPs | |
| 140˚C | C1 | 11.0 | 12.0 |
| C2 | 9.5 | No growth on NPs | |
| C3 | No growth on NPs | No growth on NPs | |
| C4 | 12.0 | 12.0 | |
| C5 | No growth on NPs | No growth on NPs |