Instrument | Shimadzu HPLC | ||
Column | YMC Triart C18 column (250 × 4.6 mm, 5 mm) | ||
Column Temp. | 30˚C | ||
Mobile phase | Time (min) | A: 0.1% TFA, water | B: 0.1% TFA, ACN |
(Isocratic mode) | 0 | 5 | 95 |
| 15 | 5 | 95 |
Detector | PDA (214 nm) | ||
Flow rate | 0.8 mL/min | ||
Injection volume | 20 mL |