Marker

Primer pair name

R Gene

Primers 5’à3’

Annealing Temperature

(Tm)

Reference

F02005

(R)

F02006

(R)

Prospect

(S)

PR90-50-32

(S)

1

BLB1F/

BLB1R

Rpi-blb1

F: AACCTGTATGGCAGTGGCATG

R: GTCAGAAAAGGGCACTCGTG

58

[18]

+

+

−

−

2

1/1’

Rpi-blb1

F: CACGAGTGCCCTTTTCTGAC

R: ACAATTGAATTTTTAGACTT

50

[19]

−

−

−

−

3

1521/518

Rpi-blb1

F: GAAAGTCTAGAGTTACACTGG

R: CAATCACAATGGCAGGAACC

58

[18]

+

+

−

−

4

517/1519

Rpi-blb1

F: CATTCCAACTAGCCATCTTGG

R: TATTCAGATCGAAAGTACAACG

58

[18]

+

+

−

−

5

BLB1F1/

BLB1R1

Rpi-blb1

F: GWGMATGGGAACATGTGAGAGC

R: GYTGTTAGGTGCTGCAATCC

55

[18]

+

+

+

+

6

RGA1F/ RGA1R

Rpi-blb1

F: CAGTCACTTTCTTGTTTGCCG

R: CAGTAGTGAAGTCACTGTGTG

55

[18]

+

+

+

+

7

CT88F1/ CT88R

Rpi-blb1

F: TTCAAGAGCTTGAAGACATAACA

R: ATGGCGTGATACAATCCGAG

60

[8]

+

+

+

+

8

BLB1F1/ BLB1R

Rpi-blb1

F: GWGMATGGGAACATGTGAGAGC

R: GTCAGAAAAGGGCACTCGTG

55

[18]

+

+

−

−

9

BLB1F/ BLB1R1

Rpi-blb1

F: AACCTGTATGGCAGTGGCATG

R: GYTGTTAGGTGCTGCAATCC

55

[18]

+

+

+

+

10

BLB2F/ BLB2R

Rpi-blb2

F: GGACTGGGTAACGACAATCC

R: GCATTAGGGGAACTCGTGCT

58

[18]

−

−

−

−

11

BLB2F1/ BLB2R1

Rpi-blb2

F: ATTGCTGGARTCATTGCTGG

R: ATTTATGGCTGCAGAGGACC

55

[18]

+

+

+

+

12

BLB2F1/ BLB2R

Rpi-blb2

F: ATTGCTGGARTCATTGCTGG

R: GCATTAGGGGAACTCGTGCT

55

[18]

ND

ND

ND

ND

13

BLB2F/ BLB2R1

Rpi-blb2

F: GGACTGGGTAACGACAATCC

R: ATTTATGGCTGCAGAGGACC

55

[18]

ND

ND

ND

ND

14

14L24LF/ 14L24LR

Rpi-blb2

F: AGAAAGCTCACCAGTGGACC

R: ATTTATGGCTGCAGAGGACC

60

[15]

+

+

+

+

15

BLB2Fa/ BLB2Ra

Rpi-blb2

F: TGTCTTTCTCAAACCTCTGG

R: AGTGAATCGGATGTCAGAGG

55

This study

+

+

+

+

16

BLB3Fa/ BLB3Rb

Rpi-blb3

F: GTCCAAGAAGCCACTCCATA

R: CAGGGTCAACATCTTTCCAC

54

This study

+

+

+

+

17

BLB3Fb/ BLB3R

Rpi-blb3

F: TGTCGCTGAAAGAGTAGRCS

R: TATGGAGTGGCTTCTTGAAC

54

This study

−

−

−

−

18

BLB3Fa/

BLB3Rc

Rpi-blb3

F: GTCCAAGAAGCCACTCCATA

R: CAGSGTCAACATCTTTCCAC

54

This study

+

+

+

+

19

BLB3F/ BLB3R

Rpi-blb3

F: TGTCGCTGAAAGAGTAGGCC

R: TATGGAGTGGCTTCTTGAAC

50

[6]

+

+

+

+

20

BLB3F/ BLB3Ra

Rpi-blb3

F: TGTCGCTGAAAGAGTAGGCC

R: TCCTGAGGTTTATATGACTCATC

50

[6]

−

−

−

−

21

Th21F/ Th21R

Rpi-blb3

F: ATTCAAAATTCTAGTTCCGCC

R: AACGGCAAAAAAGCACCAC

56

[16]

−

−

−

−

22

BT1F/

BT1R

Rpi-bt1

F: CTACATGGCTGTCATTCACT

R: CATAGGGCAACATTTAATCTC

53

This study

+

+

−

−

23

BT1F1/ BT1R1

Rpi-bt1

F: GAGATTAAATGTTGCCCTATG

R: GTTGGACAAAACTCAACTGAT

53

This study

+

+

−

−

24

BT1F/

BT1R1

Rpi-bt1

F: CTACATGGCTGTCATTCACT

R: GTTGGACAAAACTCAACTGAT

53

This study

+

+

−

−