Protein constructs

ERAP1_R-His6

ERAP1_R-IINFEKL

Method

Vapor diffusion

Vapor diffusion

Plate type

24 Well hanging-drop plate

24 Well hanging-drop plate

Temperature (K)

277

277

Protein concentration (mg∙ml−1)

5

5

Buffer composition of protein solution

Composition of reservoir solution

Volume and ratio of drop

Volume of reservoir (μl)

10 mM Tris, pH 7.5, 10 mM NaCl

100 mM Tris, pH 8.5 and 8% PEG8000

2 μl, 1:1

800

10 mM Tris, pH 7.5, 10 mM NaCl

100 mM Tris, pH 8.5 and 16% PEG8000

2 μl, 1:1

800