Time (min) | Mobile Phase A (%) | Mobile Phase B (%) |
0 - 10 | 93 - 83 | 7 - 17 |
10 - 16 | 83 - 79 | 17 - 21 |
16 - 32 | 79 - 71 | 21 - 29 |
32 - 40 | 71 - 65 | 29 - 35 |
40 - 44 | 65 - 28 | 35 - 72 |
44 - 45 | 28 - 25 | 72 - 75 |