Gene | Primers (5’ - 3’) | Accession number | Location (nt) | Product size | Exonboundary | Efficiency | Reference |
IFN-γ | Forward: CAGAGCCAAATTGTCTCCTTC | NM_174086 | 298 - 465 | 167 | 3/4 | 98.02 | Puech et al. (2015) |
Reverse: ATCCACCGGAATTTGAATCAG | |||||||
TNF-α | Forward: CCAGAGGGAAGAGCAGTCC | NM_173966 | 367 - 478 | 111 | 3/4 | 98.12 | Puech et al. (2015) |
Reverse: GGCTACAACGTGGGCTACC | |||||||
IL-10 | Forward: CTTTAAGGGTTACCTGGGTTGC | NM_174088 | 219 - 480 | 239 | 2/3 | 98.01 | Puech et al. (2015) |
Reverse: CTCACTCATGGCTTTGTAGACAC | |||||||
IL-12B | Forward: CAGCAGAGGCTCCTCTGAC | NM_174356 | 474 - 711 | 237 | 3/4 | 97.99 | Puech et al. (2015) |
Reverse: GTCTGGTTTGATGATGTCCCTG | |||||||
iNOS | Forward: CACCTCTACTGGGAGGAGATGC | DQ676956.1 | 3067 - 3169 | 102 | 5/6 | 99.905 | This study |
Reverse: GAACATAGACCTTGGGCTGGTC | |||||||
GAPDH | Forward: GGCGTGAACCACGAGAAGTATAA | NM_001034034.2 | 465 - 583 | 119 | [6]-[7] | 97.97 | Robinson et al. (2007) |
Reverse: CCCTCCACGATGCCAAAGT | |||||||
YWHAZ | Forward: GAAAGGGATTGTGGACCAG | NM_174814 | 467 - 650 | 183 | 4/5 | 98 | Puech et al. (2015) |
Reverse: GGCTTCATCAAATGCTGTCT | |||||||
ACTB | Forward: AGCAAGCAGGAGTACGATGAGT | NM_173979 | 1164 - 1402 | 244 | [6] | 98.01 | Robinson et al. (2007) |
Reverse: ATCCAACCGACTGCTGTCA |