| Parasite target | Primer designation | Primer sequences 5’-3’ | References |
| T. brucei | TBR 1 | CGAATGAATAAACAATGCGCAGT |
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| TBR 2 | AGAACCATTTATTAGCTTTGTGC | Masiga et al. (1992) |
| T. congolense (s) | TCS 1 | AGA ACC ATT TAT TAG CTT TGT GC |
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| TCS 2 | CGAGCGAGAACGGGCAC | Majiwa and Otieno (1990) |
| T. congolense (f) | TCF 1 | GGACACGCCAGAAGGTACTT |
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| TCF 2 | GTTCTCGCACCAAATCCAAC | Masiga et al. (1992) |
| T. congolense (k) | TCK 1 | GTGCCCAAATTTGAAGTGAT |
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| TCK 2 | ACTCAAAATCGTGCACCTCG | Masiga et al. (1992) |
| T. vivax | ILO 1264 | CAGCTCGCCGAAGGCCACTTGGCTGGG |
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| ILO 1265 | TCGCTACCACAGTCGCAATCGTCGTCTCAAGG | Masake et al. (1997) |