| Hemoparasite | Assay | Primer name | Primer sequence | Amplicon size (bp) |
| B. bovis | PCR | BOF | CACGAGGAAGGAACTACCGATGTTGA | 356 |
| BOR | CCAAGGAGCTTCAACGTACGAGGTCA | |||
| nPCR | BOFN | TCAACAAGGTACTCTATATGGCTACC | 291 | |
| BORN | CTACCGAGCAGAACCTTCTTCACCAT | |||
| B. bigemina | PCR | KB-16 | CATCAGCTTGACGGTAGGG | 595* |
| KB-17 | GTCCTGGCAAATGCTTTC | |||
| nPCR | KB-18 | GATGTACAACCTCACCAGAGTACC | 262 | |
| KB-19 | CAACAAAATAGAACCAAGGTCCTA | |||
| B. bovis | qPCR | CBOSG-1 | TGTTCCTGGAAGCGTTGATTC | 88 |
| CBOSG-2 | AGCGTGAAAATAACGCATTGC | |||
| B. bigemina | qPCR | CBISG-1 | TGTTCCAGGAGATGTTGATTC | 88 |
| CBISG-2 | AGCATGGAAATAACGAAGTGC |