| Target | Primer sequence (5’ to 3’) | TAa (˚C) | Size (bp) | |
| Multiplex PCR | ||||
| blaTEM | F | GAAGATCAGTTGGGTGCACGAGT | 59 | 520 |
| R | CAACTTTATCCGCCTCCATCCAGT | |||
| blaSHV | F | AACGGAACTGAATGAGGCGCT | 59 | 141 |
| R | TCCACCATCCACTGCAGCAGCT | |||
| blaCTX-M-3 | F | AATCACTGCGCCAGTTCACGCT | 59 | 479 |
| R | GAACGTTTCGTCTCCCAGCTGT | |||
| blaCTX-M-14 | F | TACCGCAGATAATACGCAGGTG | 59 | 355 |
| R | CAGCGTAGGTTCAGTGCGATCC | |||
| blaDHA | F | AACTTTCACAGGTGTGCTGGGT | 59 | 405 |
| R | CCGTACGCATACTGGCTTTGC | |||
| blaVIM | F | GTTTGGTCGCATATCGCAAC | 57 | 147 |
| R | CTTYTCAATCTCCGCGAGAAG | |||
| blaIPM-1 | F | GGAATAGAGTGGCTTAATTCTCAATC | 57 | 272 |
| R | GCTTCTAAATTTGCGTCACCC | |||
| blaIPM-2 | R | GYAACCAAACCACTACGTTATCT | 57 | 193 |
| blaSPM | F | ATGAACTCACCTAAATCGAGAGC | 57 | 340 |
| R | GTGCCGTCCAAATGAAAGTG | |||
| blaSIM | F | GGCTTAGTAGTTCTTGACAATCAC | 57 | 516 |
| R | CAATAGTGATGCGTCTCCGA | |||
| blaGIM | F | TGTAGCGTTGCCAGCTTTAG | 57 | 431 |
| R | CAGCACCTGGATAGTAGAGC | |||
| blaKHM | F | CGTTTGGTCTGCTGTTGTTTAC | 57 | 322 |
| R | GGAATGGACTTGGAGTTGAGAA | |||
| blaNDM | F | ACTTATGCCAATGCGTTGTC | 57 | 225 |
| R | TCTGTCCTTGATCAGGCAG | |||
| Single PCR | ||||
| KP16S | F | AGCACAGAGAGCTTG | 50 | 126 |
| R | ACTTTGGTCTTGCGAC | |||
| blaCMY-2 | F | CTGACAGCCTCTTTCTCCACA | 56 | 1005 |
|
| R | CTACGTAGCTGCCAAATCCAC |
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| blaKPC | F | TGTCACTGTATCGCCGTC | 54 | 1000 |
| R | CTCAGTGCTCTACAGAAAACC | |||
| OmpK35 | F | TGATCCCTGCCCTGCTGGT | 56 | 717 |
| R | CCGGAGTCATGTTGTAAGTCT | |||