Genes

Primers

Primer sequence (5’-3’)

PCR conditions

Amplicons

References

16SrRNA

Hpx1^a

CTGGAGARACTAAGYCCTCC

94˚C, 1 min; 59˚C, 1 min; 72˚C, 1 min

(40 cycles)

150 bp

Scholte et al., 1997

Hpx2

GAGGAATACTCATTGCGAAGGCGA

vacA s1/s2

SA

ATGGAAATACAACAAACACAC

94˚C, 45 s; 54˚C, 45 s; 72˚C, 45 s

(40 cycles)

s1: 176 bp

s2: 203 bp

Atherton et al., 1995

SC^a

CCTGARACCGTTCCTACAGC

Van Doorn et al., 1998

vacA m1/m2

MA^a

CACAGCCACTTTYAATAACGA

94˚C, 45 s; 55˚C, 45 s; 72˚C, 1 min

(35 cycles)

m1: 400 bp

m2: 475 bp

Van Doorn et al., 1998

MB

CGTCAAAATAATTCCAAGGG

cagA

Cag1

ATGACTAACGAAACTATTGATC

94˚C, 1 min; 53˚C, 1 min; 72˚C, 1 min

(40 cycles)

232 bp

Rasmussen et al., 2010

Cag2

CAGGATTTTTGATCGCTTTATT

dupA

Dupa1

CGTGATCAATATGGATGCTT

94˚C, 45 s; 52˚C, 45 s; 72˚C, 45 s

(35 cycles)

197 bp

Gomes et al., 2008

Dupa2

TCTTTCTAGCTTGAGCGA

oipA

HPO638F

GTTTTTGATGCATGGGATTT

94˚C, 45 s; 57˚C, 45 s; 72˚C, 45 s

(35 cycles)

401 bp

Ben Mansour et al., (2010)

HPO638R

GTGCATCTCTTATGGCTTTG