Enzyme | pNP-C8 |
| pNP-C10 |
| pNP-C12 |
| ||||||
Km (μM) | kcat (min−1) | kcat/Km (min−1・μM−1) | Vmax (μM・min−1) | Km (μM) | kcat (min−1) | kcat/Km (min−1・μM−1) | Vmax (μM・min−1) | Km (μM) | kcat (min−1) | kcat/Km (min−1・μM−1) | Vmax (μM・min−1) | |
Wild-type | 69.30 ± 8.61 | 989.70 ± 36.33 | 14.28 | 0.004 | 395.33 ± 62.88 | 864.09 ± 27.11 | 2.19 | 0.018 | 237.59 ± 13.07 | 259.35 ± 0.59 | 1.09 | 0.011 |
L278G | 81.09 ± 2.65 | 65.72 ± 7.53 | 0.81 | 0.004 | 475.24 ± 40.21 | 1965.87 ± 183.98 | 4.14 | 0.027 | 129.53 ± 7.79 | 486.99 ± 21.13 | 3.76 | 0.007 |
L278P | 69.70 ± 9.51 | 447.68 ± 30.76 | 6.42 | 0.005 | 2684.66 ± 123.63 | 2048.63 ± 151.33 | 0.76 | 0.133 | 288.00 ± 31.55 | 257.01 ± 4.82 | 0.89 | 0.017 |