Enzyme

pNP-C8

pNP-C10

pNP-C12

Km (μM)

kcat (min−1)

kcat/Km

(min−1・μM−1)

Vmax

(μM・min−1)

Km (μM)

kcat (min−1)

kcat/Km

(min−1・μM−1)

Vmax

(μM・min−1)

Km (μM)

kcat (min−1)

kcat/Km

(min−1・μM−1)

Vmax

(μM・min−1)

Wild-type

69.30 ± 8.61

989.70 ± 36.33

14.28

0.004

395.33 ± 62.88

864.09 ± 27.11

2.19

0.018

237.59 ± 13.07

259.35 ± 0.59

1.09

0.011

L278G

81.09 ± 2.65

65.72 ± 7.53

0.81

0.004

475.24 ± 40.21

1965.87 ± 183.98

4.14

0.027

129.53 ± 7.79

486.99 ± 21.13

3.76

0.007

L278P

69.70 ± 9.51

447.68 ± 30.76

6.42

0.005

2684.66 ± 123.63

2048.63 ± 151.33

0.76

0.133

288.00 ± 31.55

257.01 ± 4.82

0.89

0.017