Primer sequence, PCR preparations and conditions used in molecular characterization and genotyping of Salmonella enterica obtained in the study. | |||
Target primer sequence | PCR preparation (25 µl) | PCR conditions | Reference(s) |
InvA SAL1 | 12.5 µl Dream Taq mastermix, | 5 min at 94˚C prior to | [12] |
SAL2 | 8.5 µl nuclease free water, 3 µl | 35 cycles of 1 min at 94˚C annealing |
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| Template DNA and 0.5 µl of each | Temperature at 55˚C for 1 min at 72˚C. |
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| Primer mix. |
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Stn P15 | 12.5 µl Dream Taq mastermix, | 5 min at 94˚C prior to 35 cycles of | [12] |
Stn M13 | 8.5 µl nuclease free water 3 µl | 1 min at 94˚C annealing temperature |
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| Template DNA and 0.5 µl of each | At 60˚C for 90 min, extension at 72˚C |
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| Primer mix. | For 1 min and a final extension of 10 min at 72˚C. |
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ERIC1 | 12.5 µl Dream Taq mastermix, | 1 min at 94˚C prior to 1 min at 95˚C | [12] |
ERIC2 | 8.5 µl nuclease free water 3 µl | Annealing temperature at 52˚C for |
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| Template DNA and 0.5 µl of each | 1 min extension at 65˚C for 8 min |
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| Primer mix. | And a final extension of 16 min at 65˚C. |
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QRDR gyrAF | 12.5 µl Dream Taq mastermix, | 5 min at 94˚C prior to 35 cycles of | [13] |
gyrAR | 8.5 µl nuclease free water 3 µl | 1 min at 94˚C, annealing temperature |
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| Template DNA and 0.5 µl of each | At 54˚C for 1 min extension at 72˚C. |
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| primer mix. | For 1 min and a final extension of 10 min at 72˚C. |
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pCT(008)F | 12.5 µl Dream Taq mastermix, | 5 min at 94˚C prior to 35 cycles of | [13] |
pCT(009)R | 8.5 µl nuclease free water, 3 µl | 1 min at 94˚C annealing temperature |
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| Template DNA and 0.5 µl each | At 60˚C for 1 min. extension at 72˚C |
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| Primer mix. | For 1 min and a final extension of 10 min at 72˚C. |
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