Primer sequence, PCR preparations and conditions used in molecular characterization and genotyping of Salmonella enterica obtained in the study.

Target primer sequence

PCR preparation (25 µl)

PCR conditions

Reference(s)

InvA SAL1

12.5 µl Dream Taq mastermix,

5 min at 94˚C prior to

[12]

SAL2

8.5 µl nuclease free water, 3 µl

35 cycles of 1 min at 94˚C annealing

Template DNA and 0.5 µl of each

Temperature at 55˚C for 1 min at 72˚C.

Primer mix.

Stn P15

12.5 µl Dream Taq mastermix,

5 min at 94˚C prior to 35 cycles of

[12]

Stn M13

8.5 µl nuclease free water 3 µl

1 min at 94˚C annealing temperature

Template DNA and 0.5 µl of each

At 60˚C for 90 min, extension at 72˚C

Primer mix.

For 1 min and a final extension of 10 min at 72˚C.

ERIC1

12.5 µl Dream Taq mastermix,

1 min at 94˚C prior to 1 min at 95˚C

[12]

ERIC2

8.5 µl nuclease free water 3 µl

Annealing temperature at 52˚C for

Template DNA and 0.5 µl of each

1 min extension at 65˚C for 8 min

Primer mix.

And a final extension of 16 min at 65˚C.

QRDR gyrAF

12.5 µl Dream Taq mastermix,

5 min at 94˚C prior to 35 cycles of

[13]

gyrAR

8.5 µl nuclease free water 3 µl

1 min at 94˚C, annealing temperature

Template DNA and 0.5 µl of each

At 54˚C for 1 min extension at 72˚C.

primer mix.

For 1 min and a final extension of 10 min at 72˚C.

pCT(008)F

12.5 µl Dream Taq mastermix,

5 min at 94˚C prior to 35 cycles of

[13]

pCT(009)R

8.5 µl nuclease free water, 3 µl

1 min at 94˚C annealing temperature

Template DNA and 0.5 µl each

At 60˚C for 1 min. extension at 72˚C

Primer mix.

For 1 min and a final extension of 10 min at 72˚C.