| Time (min) | Mobile Phase A (%, v/v) | Mobile Phase B (%, v/v) |
| T0.0 | 50 | 50 |
| T5.0 | 50 | 50 |
| T35.0 | 5 | 95 |
| T40.0 | 5 | 95 |
| T40.1 | 50 | 50 |
| T45.0 | STOP | -- |