Compound | Retention time (min) | Quality control concentration (ng/mL) | Matrix effect (%) | Extraction recovery (%) | LOD (ng/mL) |
(-)-Cotinine | 4.6 | 1 | 97.9 | 102.7 | 0.1 |
10 | 98.9 | 73.6 | |||
100 | 100.2 | 79.4 | |||
(-)-Nicotine | 4.4 | 1 | 97.0 | 69.1 | 0.2 |
10 | 95.5 | 73.0 | |||
100 | 100.4 | 70.9 | |||
(+)-Bicuculline | 7.5 | 1 | 93.2 | 106.1 | 0.02 |
10 | 95.2 | 101.3 | |||
100 | 93.5 | 97.7 | |||
14-Anisoylaconine | 9.7 | 2 | 83.8 | 81.8 | 0.4 |
19 | 76.8 | 70.5 | |||
189 | 74.5 | 74.5 | |||
Aconitine | 11.5 | 1 | 56.0 | 59.8 | 0.2 |
10 | 59.2 | 55.2 | |||
100 | 59.4 | 51.8 | |||
Atropine | 6.9 | 1 | 102.4 | 94.8 | 0.01 |
10 | 101.2 | 86.7 | |||
100 | 100.4 | 84.5 | |||
Benzoylhypaconine | 9.5 | 1 | 85.0 | 85.3 | 0.5 |
9 | 78.8 | 84.7 | |||
94 | 76.1 | 88.8 | |||
Benzoylmesaconine | 9.0 | 2 | 90.9 | 84.9 | 0.4 |
19 | 83.8 | 88.2 | |||
188 | 80.5 | 94.1 | |||
Cocaine | 8.9 | 1 | 93.1 | 81.8 | 0.05 |
10 | 92.5 | 89.4 | |||
100 | 95.3 | 85.2 | |||
Colchicine | 7.6 | 1 | 94.2 | 94.0 | 0.05 |
10 | 94.1 | 85.4 | |||
100 | 90.8 | 78.1 | |||
Ephedrine | 5.4 | 1 | 102.7 | 72.6 | 0.2 |
10 | 102.0 | 77.7 | |||
100 | 99.9 | 79.6 | |||
Galantamine | 5.3 | 1 | 96.3 | 102.1 | 0.02 |
10 | 98.1 | 100.4 | |||
100 | 98.9 | 99.4 | |||
Galantamine-d6 | 5.3 | - | - | - | - |
Hypaconitine | 11.6 | 3 | 56.9 | 68.0 | 0.6 |
30 | 56.7 | 52.4 | |||
300 | 55.6 | 50.4 | |||
Jesaconitine | 11.6 | 1 | 58.8 | 57.8 | 0.5 |
10 | 65.2 | 50.3 | |||
100 | 63.5 | 48.5 | |||
Lycorine | 4.1 | 1 | 99.4 | 95.6 | 0.02 |
10 | 97.6 | 93.5 | |||
100 | 99.1 | 90.5 | |||
Mesaconitine | 11.2 | 2 | 53.1 | 65.2 | 0.4 |
20 | 56.2 | 68.2 | |||
200 | 55.4 | 69.6 | |||
Morphine | 3.9 | 1 | 113.3 | 90.6 | 0.2 |
10 | 99.3 | 83.4 | |||
100 | 104.0 | 75.8 | |||
Papaverine | 9.9 | 1 | 72.6 | 62.2 | 0.02 |
10 | 79.2 | 53.4 | |||
100 | 79.4 | 53.5 | |||
Pilocarpine | 4.7 | 1 | 95.6 | 98.4 | 0.1 |
10 | 98.9 | 89.5 | |||
100 | 98.2 | 91.3 | |||
Protopine | 9.1 | 1 | 87.8 | 85.1 | 0.2 |
10 | 91.1 | 81.9 | |||
100 | 91.4 | 85.2 | |||
Reserpine | 10.6 | 1 | 72.0 | 40.0 | 0.2 |
10 | 70.6 | 54.5 | |||
100 | 71.5 | 51.3 | |||
Scopolamine | 5.6 | 1 | 95.9 | 111.6 | 0.01 |
10 | 97.8 | 106.6 | |||
100 | 98.2 | 103.6 | |||
Strychnine | 7.6 | 1 | 103.0 | 102.2 | 0.05 |
10 | 101.0 | 98.0 | |||
100 | 97.8 | 92.1 |