S. No

Concentration (µg/ml)

Flow rate (ml/min)

Mobile Phase ratio (ACN:Water)

Rt

Peak area (Mean)

% RSD

1.

13

1

79:21

3.2

53,580

0.3

2.

13

1

81:19

3.2

53,525

0.16

3.

13

0.9

80:20

3.2

53,484

0.1

4.

13

1.1

80:20

3.2

53,564

0.16