| Parameters | Description | Units |
| [Et] | Total active enzyme concentration in the matrix | mmol/L |
| [Eox] | Enzyme concentration of the oxidized mediator | mmol/L |
| [ES] | Enzyme concentration of the substrate | mmol/L |
| [Ered] | Reduced enzyme concentration | mmol/L |
| [O2] | Concentration of oxygen at any position in the enzyme layer | mmol/L |
| [O2]b | Oxygen concentration in the bulk electrolyte | mmol/L |
| [S] | Concentration of substrate at any position in the enzyme layer | mmol/L |
| [S]b | Substrate concentration in the bulk electrolyte | mmol/L |
| [Medox] | Concentration of oxidised mediator at any position in the enzyme layer | mmol/L |
| [Medox]b | Oxidised mediator concentration in the bulk electrolyte | mmol/L |
| Do | Diffusion coefficient of oxygen | cm2∙s−1 |
| Ds | Diffusion coefficient of substrate | cm2∙s−1 |
| Dm | Diffusion coefficient of mediator | cm2∙s−1 |
| d | Enzyme layer thickness | cm |
| y | Distance from the electrode | cm |
| k1, k4, k5 | Rate constants | L∙mol−1∙s−1 |
| k2, k3 | Rate constants | s−1 |
| Ko | Partitioning coefficient for oxygen | None |
| Ks | Partitioning coefficient for substrate | None |
| Km | Partitioning coefficient for mediator | None |
| Bo | Normalized surface concentration of oxygen | None |
| Bs | Normalized surface concentration of the substrate | None |
| Bm | Normalized surface concentration of mediator | None |
| Fo | Normalized oxygen concentration | None |
| Fs | Normalized substrate concentration | None |
| Fm | Normalized mediator concentration | None |
| x | Normalized distance from the electrode | None |
| Jox | Normalized current | None |