| Time in minutes | Mobile Phase A (In %) | Mobile Phase B (In %) |
| 0.0 | 82 | 18 |
| 5.0 | 82 | 18 |
| 6.0 | 95 | 5 |
| 12.0 | 95 | 5 |
| 13.0 | 82 | 18 |
| 30.0 | 82 | 18 |