Gene

Primer name

Sequence (5' to 3')

Annealing T (˚C)

Reference

ESBLs

blaSHV

SHV-F

TTATCTCCCTGTTAGCCACC

58

[19]

SHV-R

GATTTGCTGATTTCGCTCGG

blaTEM

TEM-F

GCGGAACCCCTATTTG

55

[19]

TEM-R

ACCAATGCTTAATCAGTGAG

blaCTX-M1

CTX-M 1-F

GGTTAAAAAATCACTGCGTC

56

[19]

CTX-M1-R

TTGGTGACGATTTTAGCCGC

blaCTX-M2

CTX-M2-F

GATGAGACCTTCCGTCTGGA

61

[19]

CTX-M2-R

CAGAAACCGTGGGTTACGAT

blaCMY-2

CMY-2 F

ATGATGAAAAAATCGTTATGCTGC

62

[19]

CMY-2 R

GCTTTTCAAGAATGCGCCAGG

AmpC

AmpC-F

ATGATGAAAAAATCGTTATGC

56

[19]

AmpC-R

TTGCAGCTTTTCAAGAATGCGC

ExPEC-associated virulence genes

fimC

fimC-F

TAAGGAAATCGCAGGAA

52.0

[20]

fimC-R

GTCAGGTAATAGGGTGTCG

ompA

ompA-F

TTTTGGATGATAACGAGG

50.0

[20]

ompA-R

TGCTGGGTAAGGAATAAC

betA

betA-F

CGGTTTCGGCGTGGTTAC

60.0

[20]

betA-R

GTGGGCATGTGCGGATTA

ibeA

ibeA-F

AGGCAGGTGTGCGCCGCGTAC

58.0

[20]

ibeA-R

TGGTGCTCCGGCAAACCATGC

rfaH

rfaH-F

TGTATCGCAACACGCACG

54.0

[20]

rfaH-R

AAAACCAGTCGAGCCTTC

irp2

irp2-F

AAGGATTGCCTGTTACCGGAC

52.0

[20]

irp2-R

TCGTCGGGCAGCGTTTCTTCT

fyuA

fyuA-F

GCTTTATCCTCTGGCCTT

52.0

[20]

fyuA-R

GGCATATTGACGATTAAC