Components

Initial concentration

Final concentration

Reaction volume (μL)

Buffer taq

10×

0.25×

5

Primer F

20 Μm

0.50 μM

1

Primer R

20 Μm

0.50 μM

1

d NTP

10 Μm

0.25×

1

H₂O

-

-

39.5

Taq polymerase

5 U/Μl

0.13

0.5

ADN

-

-

2

Final volume of reaction 50 μL