Date

Treatment

Con

ConN

Str

StrN

Man

ManN

Microbial biomass (mg・C・kg−1・soil)

Con

ConN

Str

StrN

Man

ManN

Sowing

29.72 (0.87)

25.88 (1.81)

41.6 (2.84)

38.44 (1.42)

49.88 (1.07)

49.88 (1.41)

6 weeks

33.05 (1.77)

31.00 (1.27)

47.11 (2.43)

42.38 (2.78)

52.55 (1.44)

51.51 (1.33)

Flowering

17.27 (2.72)

26.16 (2.10)

11.61 (1.18)

27.55 (3.62)

15.33 (3.69)

19.77 (2.08)

Harvested

4.94 (0.44)

5.88 (0.67)

6.16 (0.65)

4.16 (0.30)

6.5 (0.70)

8.6 (0.87)

Phosphatase (µg・PNP・g−1・soil・h−1)

Sowing

85.87 (2.0)

92.23 (4.5)

119.29 (4.0)

127.72 (4.2)

127.75 (5.0)

146.03 (4.0)

6 weeks

85.34 (4.9)

85.73 (2.5)

109.34 (2.8)

112.42 (4.5)

109.35 (1.3)

141.89 (5.4)

Flowering

91.02 (4.6)

102.86 (7.7)

113.33 (5.4)

146.80 (7.6)

124.53 (7.4)

158.89 (7.1)

Harvested

73.20 (2.8)

91.96 (4.3)

104.17 (3.4)

109.75 (2.8)

114.16 (4.3)

131.75 (3.4)

B-glucosidase (µg・PNP・g・−1・soil・h−1)

Sowing

9.87 (0.7)

6.70 (0.6)

14.55 (0.5)

12.49 (0.3)

15.71 (0.4)

15.62 (0.5)

6 weeks

8.41 (0.6)

7.24 (1.0)

14.09 (0.6)

14.34 (0.6)

18.05 (0.7)

16.85 (0.7)

Flowering

9.82 (0.8)

6.21 (0.5)

14.46 (0.8)

19.76 (0.8)

22.13 (1.3)

18.97 (0.6)

Harvested

16.84 (0.9)

21.27 (2.0)

17.29 (0.7)

16.95 (1.3)

24.61 (0.7)

19.30 (0.7)

Fluorescein diacetate (µg・fl・g−1・soil・h−1)

Sowing

0.86 (0.0)

0.81 (0.0)

1.32 (0.0)

1.80 (0.1)

1.37 (0.0)

2.07 (0.0)

6 weeks

1.35 (0.1)

1.85 (0.1)

2.65 (0.1)

3.25 (0.1)

3.65 (0.1)

3.79 (0.1)

Flowering

1.30 (0.0)

1.75 (0.0)

1.63 (0.1)

2.70 (0.1)

2.94 (0.2)

3.20 (0.2)

Harvested

1.17 (0.0)

1.74 (0.1)

1.84 (0.1)

1.98 (0.1)

2.51 (0.1)

2.59 (0.0)

Source of variation

df

Microbial Biomass

Phosphatase

B-glucosidase

Fluorescein diacetate

P (<0.05)

Date

3

<0.0001

0.037

<0.0001

<0.0001

Input management

2

0.000

<0.0001

<0.0001

<0.0001

N fertilizer

1

ns

0.002

ns

0.002

Date*input management

6

0.001

ns

0.024

ns

Date*N fertilizer

3

ns

ns

ns

ns

Input management*N fertilizer

2

ns

ns

ns

ns

Date*input management*N fertilizer

6

ns

ns

ns

ns