Compound

Without protein

With HSA (c = 10 μg/ml)

With HSA after 1 hour (c = 10 μg/ml)

λ (nm)

A

λ (nm)

A

λ (nm)

A

1c

270

0.383

268

0.252

270

0.222

427

0.551

427

0.522

429

0.368

4a

340

0.461

335

0.432

335

0.379

4c

271

0.485

267

0.399

271

0.289

416

0.511

399

0.479

405

0.294

2b

260

0.279

260

0.333

233

0.594

382

0.460

383

0.516

394

0.127