Assay one mean Ct values with mixed targets

Target at 6.15 × 106 plasmid copies per µL

Coxiella

A. phagocytophilum

B. burgdorferi

E. chaffeensis

Coxiella

12.83 (12.35)

-

-

-

A. phagocytophilum

-

13.03 (13.13)

-

-

B. burgdorferi

-

-

11.69 (12.96)

-

E. chaffeensis

-

-

-

12.89 (12.1)

Assay two mean Ct values with mixed target

Target at 6.15 × 106 plasmid copies per µL

Rickettsia

Other non burgdorferi Borrelia

Bartonella

Babesia

Rickettsia

11.13 (10.33)

-

-

-

Other Borrelia

-

10.39 (10.88)

-

-

Bartonella

-

-

12.08 (12.1)

-

Babesia

-

-

-

24.20 (24.53)