| Decosterd & Woolf 2000 | Shields et al. 2003 | Bourquin et al. 2006 |
Species/Strain | Sprague Dawley Rats | C57BL/6 mice | C57BL/6 mice |
Gender | Male | Male & Female | Male |
Weight/Age | 200 - 250 g (age not specified) | 20 - 25 g (age not specified) | 25 - 30 g/2months old |
Anesthesia | Halothane (2%) | Ketamine & Xylazine (60 and 8 mg/kg) OR Halothane (1.75% - 2.5%) | Isoflurane (1.5% - 2.5%) |
Side of Injury | Not specified | Not specified | Left hindlimb |
Injury | Tibial ligated/peroneal ligated; both severed, 2-4mm stumps removed distal to ligations
| Tibial ligated/peroneal ligated; both severed
| Tibial + peroneal ligated together; 1 - 2 mm stumps removed distal to ligation
|
Nerve Ligation | 5-0 silk | 9-0 silk | 6-0 silk |
Muscle Suture | Not specified | 6-0 silk | 6-0 silk |
Skin Suture | Not specified | 6-0 silk | 6-0 silk & surgical micro clips |
Mechanical Allodynia | Yes | Not with Ketamine/Xylazine; Not with Halothane | Yes |
Cold Allodynia | Yes | Not determined | Not determined |