Protocols

Master mix Reagents

Vol. for 1 rxs* at 25 ul

SINE 200X

Buffer 5×

4

25 Mm MgCl2

2

5 Mm d’NTPS

0.8

Primer F (20 Um)

0.3

Primer R (20 Um)

0.3

Taq DNA polymerase (5 U/ul)

0.07

D dH2O

10.53

DNA template (1/10 CTAB)

2

Favia

Master mix Reagents

Vol. for 1 rxs at 25 ul

Buffer 5×

5

25 Mm MgCl2

1

5 Mm d’NTPS

1

R3 (10 Um)

0.75

R5 (10 Um)

0.75

B/S (10 Um)

1.75

Mop (10 Um)

1.75

Taq DNA polym (5 U/ul)

0.05

D dH2O

11.95

DNA template (1/10 CTAB)

1.5

Scott

Master mix Reagents

Vol. for 1 rxs at 25 ul

5× Buffer

5

25 Mm MgCl2

1.5

5 Mm d’NTPS

1

UN (10 Um)

0.5

AG (10 Um)

0.5

AR (10 Um)

0.5

Taq DNA polym (5 U/ul)

0.05

D dH2O

13.95

DNA template (1/10 CTAB)

2

Cohuet

Master mix Reagents

Vol. for 1 rxs at 25 ul

5× £Buffer

5

25 Mm MgCl2

1

5 Mm d’NTPS

1

FUN F (10 µM)

0.5

FUN R (10 µM)

0.5

Taq DNA polym (5 U/ul)

0.1

D dH2O

13.9

DNA template (1/10 CTAB)

3