Method | Sitting-drop vapour diffusion |
Plate type | 48-well plates |
Temperature (˚C) | 18 |
Protein concentration (mg∙ml−1) | 10 |
Buffer composition of protein solution | 20 mM Tris-HCl at pH 8.0, 150 mM NaCl |
Composition of reservoir solution† | 1.6 M sodium citrate tribasic dehydrate at pH 6.5. |
Volume and ratio of drop | 1.0 μl, 1:1 ratio of protein: reservoir solution |
Volume of reservoir | 70 μl |