Enzyme Species | Kd1 Kd2 | ||||||
Zn(II) nM | Cd(II) nM | Co(II) µM | Zn(II) µM | Cd(II) µM | Co(II) µM | ||
Wild type | 0.62 (±0.08) | 8.3 (±0.5) | 0.093 (±0.015) | 1.50 (±0.71) | 5.9 (±1.0) | 66.7 (±10.0) | |
H86S | 5.30 (±2.34) | ND | 10.5 (±1.5) | 0.32 (±0.11) | ND | ND | |
H88S | 0.38 (±0.15) | 3.8 (±0.3) | 9.1 (±1.1) | 1.13 (±0.19) | 1.4 (±0.1) | ND | |
H149S | 3.11 (±0.05) | ND | 2.7 (±0.3) | 0.19 (±0.02) | ND | ND | |
D90N | 2.00 (±0.39) | ND | 20.0 (±3.5) | 5.02 (±2.39) | ND | ND | |
C168S | 0.61 (±0.19) | ND | 3.1 (±0.4) | 2.34 (±0.59) | ND | ND | |
C168A | ND | ND | 1.1 (±0.1) | ND | ND | ND | |
H210S | 0.43 (±0.11) | ND | 0.35 (±0.05) | 2.53 (±0.5) | ND | ND |