| LC-MS/MS | SHIMADZU LCMS/MS - 8040. |
| Detector | Mass Spectrophotometer |
| Column | Kinetex, 2.6 µ, C18 Column, 100 × 3.0. |
| Column oven temperature | 40˚C |
| Retention time | Tebuconazole - 5.2 min Fluopyram - 5.4 min |
| Nebulizing gas | Nitrogen |
| Nebulizing gas flow | 2.0 litres/min |
| Pump mode/flow | Gradient/0.4 ml/min |
| Solvents | A: Ammonium Formate in Water (10 Mm) B: Ammonium Formate in Acetonitrile (10 Mm) |
| LC programme | Time solvent Conc. 0.01 B Conc. 50% 1.00 B Conc. 80% 4.00 B Conc. 50% 8.00 B Conc. 50% |
| Total Time Programme | 8 min |